FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005398976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005398976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61601324
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC786770112.771967368753309TruSeq Adapter, Index 2 (100% over 50bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC3671230.5959660867029416TruSeq Adapter, Index 2 (98% over 50bp)
GCTCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC2907210.4719395316892864TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATGTATCTCGTATGC1468500.2383877333545623TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1460260.23705009976733615TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCGATGTATCTCGTATGC1323930.21491908193401818TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCGATGTATCTCGTATGC1239200.2011645074381843TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCGATGTATCTCGTATGC1179360.19145043051347402TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCGATGTATCTCGTATGC1148770.18648462815507016TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCGATGTATCTCGTATGC1133240.1839635784451646TruSeq Adapter, Index 2 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC891160.14466572179520037TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC774140.12566937684651064TruSeq Adapter, Index 2 (98% over 50bp)
GTTCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC734520.11923769690404706TruSeq Adapter, Index 2 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)