FastQCFastQC Report
Tue 23 May 2023
EGAF00005398988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005398988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45818190
Sequences flagged as poor quality0
Sequence length126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCACTTCACGTGTAGATCT22314864.870305876334268Illumina Single End PCR Primer 1 (96% over 32bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT838430.18299064192627426No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC655510.1430676331823671No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG638570.139370411620363No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)