FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37901460
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATTA340800.023.01431512
GGATTAC323350.022.81664713
GATTACA336400.022.12925314
ATTACAG366200.020.07024815
TGGGATT433950.019.477511
TTACAGG387700.018.55102216
TACAGGC350550.017.2621817
AAAGTGC347400.016.7714774
CCAAAGT387800.015.557062
CGTCGAG60650.015.3003861
CTGGGAT570300.015.05393310
AAGTGCT401150.015.0042795
GGCGTGA211200.014.79813821
ACAGGCG256000.014.68300518
CAAAGTG417550.014.5492083
GTCGAGA65000.014.4368192
ACCGCGC115050.014.17583931
GCGTGAG225250.013.89062222
TGATCAA169650.013.7602789
CGTGAGC230400.013.58018523
Produced by FastQC (version 0.11.5)