FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21210502
Sequences flagged as poor quality0
Sequence length35-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1798730.8480374486186135No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1070270.5045943749940478No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG921590.43449702416284164No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT629030.29656535239005655No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC619830.2922278784349376No Hit
GTGTGGTTGGTGCGCGGACACGCACTGCCTGCGTAACTAGAGGGAGCTGA484320.22833971586339635No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC465730.2195751896866939No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC430810.20311164723965514No Hit
CTCGTATACCCTTGACCGAAGACCGGTCCTCCTCTATCGGGGATGGTCGT403800.19037738946489807No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA386310.18213147430456855No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG375730.17714337925618168No Hit
CGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCA277510.13083613013968268No Hit
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG276770.1304872463650318No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC276560.1303882388073606No Hit
GTCTGTTCCAAGCTCCGGCAAAGGAGGCATCCGCCGGGCCCCTCCCCGAA263610.12428277275097026No Hit
CTTGACCGAAGACCGGTCCTCCTCTATCGGGGATGGTCGTCCTCTTCGAC261890.12347185370718712No Hit
GTGGTTGGTGCGCGGACACGCACTGCCTGCGTAACTAGAGGGAGCTGACG257980.12162842727626154No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC252550.11906837471362063No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT246030.11599442578021019No Hit
CTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATA238310.11235471937439293No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT224980.10607009678507374No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG219670.1035666199696735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC156950.063.9656454
ACCGCGT158350.063.2231186
CCGCGTT161650.061.961167
CGCGTTC166500.060.1464048
GACCGCG172500.058.0369875
GCGTTCT179250.055.9204649
CAGACCG202500.049.8554233
GGAGTCT496600.036.4877323
TGGAGTC494300.036.474212
GAGTCTT498900.036.1501584
CTCAGAC319250.034.905181
TTGGAAG545700.033.2008139
AATACGG166850.032.35837632-33
TCAGACC318550.032.3036842
AGTCTTG576150.031.7752825
CTTGGAA580850.031.4335928
TACGGAG176750.030.67030734-35
TCTTGGA609450.030.1235527
GTCTTGG613450.029.7973886
AGAACGA184450.029.37691944-45
Produced by FastQC (version 0.11.5)