FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2050227
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT8900.0118.932581
CGTAGCG8200.0115.8231661
CCGGTAG7200.0113.784721
CCGTCGA2450.0109.489796
CGATTGA5650.0109.070811
CTCGTAC10100.0108.391091
CTCTACG9200.0106.385871
CGTTAGA6800.0105.551471
TACGGGC7850.0105.286621
ACCGTAA11850.0105.232071
CGTACGT3450.0102.9710163
CGCCGAA4750.0102.263162
CTAATGG8250.0101.93941
CTTTGCG6450.0101.162791
GCCGTAG6250.0100.923
TGACTAG7350.0100.612241
CGACTTC9850.0100.1015241
ATGCGAG3500.099.428573
TTGAACG6150.099.024381
ACAACGA4300.097.7906957
Produced by FastQC (version 0.11.5)