FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2082492
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACT8000.0112.3758
TCGACTA5100.0112.303929
AGCGTAT9850.0110.40611
TACGGGC10800.0110.092581
TTACGAG3450.0109.275363
GATGCGA3600.0108.749999
GCGCAAC7450.0108.0201341
CATCGAA7750.0107.580641
CGGACTG7850.0107.133751
CGCTATC6150.0106.097561
TGACGGC9300.0106.02151
GGTCGAC5200.0104.567317
CGATTGA3350.0103.880591
TAAACCG7550.0103.708611
TGGTCGA7150.0103.4265756
CTCGTAC6950.0103.2733841
CCGGGAT10100.0100.495051
ACTAGGT11450.0100.043673
AGTACGA6450.0100.038768
CGTACGC3050.099.836077
Produced by FastQC (version 0.11.5)