FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2082492
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC417152.0031289435925803No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC403831.9391671132470139No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC157300.755345038540364No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC152350.7315754394254576No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC41650.20000076831027444No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT33310.1599525952560682No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG81600.0105.640311
GTCTCTC57850.086.598962
TCTCTCA66100.075.461423
TCAGCTG66950.075.15317
AGTCTCT68900.073.026121
TCCGATC4600.070.923916
AGCTGGT70050.070.79239
CAGCTGG76350.066.3752448
CTTCCGA5100.065.392164
GGCTCAA148450.059.973066
CTCAAAC149950.059.5181738
GCTCAAA150950.059.027827
TCAAACA156550.057.1015669
CCGATCT5950.056.050427
ATGGCTC162100.054.6098064
CTGATGG166050.053.922011
TGGCTCA168550.052.8641365
CTCTCAG94950.052.838344
GATGGCT172950.051.4353833
ATACGGC1152.4865585E-950.4347845
Produced by FastQC (version 0.11.5)