FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2133790
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGC11050.0126.628971
GCGTATG6800.0115.1470642
TAAACCG8250.0115.121211
ATCCGAA9450.0114.3121641
TTACGCG6250.0113.689
TAGTTGG12300.0110.813011
GGTTACG8550.0109.385967
CGTAGCG6950.0107.446041
CGATTGA5300.0106.698121
CGTATGT7400.0105.8108143
AGCGTAT11950.0104.351461
CCTTACG5700.0104.298241
ATGCGAT7400.0103.851361
TCGCGAG6500.0103.730771
TACATCG5700.0103.0263066
AACGTTG7450.0102.1812061
CTCGTAC9550.0101.7277451
GCATGCG8850.0100.762711
TACTCGC5400.0100.694451
CACGCGA3250.0100.384618
Produced by FastQC (version 0.11.5)