FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2133790
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC524122.45628670112804No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC462342.166754929023006No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC211250.9900224483196565No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC181640.8512552781670174No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC52370.24543183724733925No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT44430.20822105268091048No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGCTTT23650.11083564924383375No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG23120.10835180594154063No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG88300.0112.978481
GTCTCTC63900.088.0438162
AGTCTCT69250.081.137191
TCTCTCA71050.079.285713
TCAGCTG75600.074.130297
AGCTGGT78250.070.9712459
CAGCTGG81900.068.959098
GGCTCAA179850.064.095086
GCTCAAA183000.063.1106577
CTCAAAC183600.063.0228738
TCAAACA192450.060.237739
TGGCTCA194600.059.3486635
ATGGCTC195700.058.9780244
CTGATGG197000.058.6256371
TCCGATC4800.057.3958326
GATGGCT201150.057.3079763
TACGGCG902.0510925E-856.388896
TGATGGC209150.055.25462
CTTCCGA5200.054.3750044
TGATACG1258.913048E-1152.23
Produced by FastQC (version 0.11.5)