FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2082866
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACG7250.0119.01
GATCGCG6200.0113.427421
CGGGATA4100.0111.4024352
CTTACCG9000.0108.749991
CGTAGCG5900.0108.13561
CACCGTA10750.0107.9069759
TCGAGCG5050.0107.673275
TACGGTA5200.0107.355771
GTCGTTC4150.0106.566265
CGATTGA7100.0106.1971741
ATCCGAA9200.0105.597831
TACTCGC8350.0105.059881
TCGAGGC16400.0104.771341
TGACGGC6800.0104.485291
CGTACGG4100.0104.329279
TCGTACG4100.0104.329278
CCGTTTG5800.0103.756
GTACGCC3500.0103.5714349
GCGCAAC9200.0103.2336961
CACACCG8100.0102.93218
Produced by FastQC (version 0.11.5)