FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2082866
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC398951.915389660208578No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC360911.7327566919811452No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC120480.5784337542597555No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC106450.5110746442642013No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC38370.1842173236300367No Hit
GTTCAGAAATTGGATCACACCTGAAAGTTACATTCTGTCCCCTCTTTGTG36410.17480721275396496No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG35180.1689018880715322No Hit
GTCAGAAACTCTGGGCCCTGCCCCAGGGTCTGTCGGTACCAATAAAGGCG34600.1661172634245314No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT32640.15670715254845968No Hit
GCTTCATTCTGGAAGTAAGTCAGAAACTCTGGGCCCTGCCCCAGGGTCTG29780.14297607239255908No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG27750.13322988612805625No Hit
GTTACATTCTGTCCCCTCTTTGTGATCTTGTGTCTGGGGTCCTGGGAGAC27340.13126144456724534No Hit
GGTACCAATAAAGGCGGTTGTGTTCAGAAATTGGATCACACCTGAAAGTT25990.12477999064750205No Hit
GAAATTGGATCACACCTGAAAGTTACATTCTGTCCCCTCTTTGTGATCTT24610.11815450441843113No Hit
GGATCACACCTGAAAGTTACATTCTGTCCCCTCTTTGTGATCTTGTGTCT24400.11714627825313774No Hit
GTCGGTACCAATAAAGGCGGTTGTGTTCAGAAATTGGATCACACCTGAAA24260.11647412747627549No Hit
CAGAAATTGGATCACACCTGAAAGTTACATTCTGTCCCCTCTTTGTGATC22740.10917649047034231No Hit
CCTGAAAGTTACATTCTGTCCCCTCTTTGTGATCTTGTGTCTGGGGTCCT21470.10307912270880605No Hit
AGAAACTCTGGGCCCTGCCCCAGGGTCTGTCGGTACCAATAAAGGCGGTT21240.1019748750039609No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG69550.0106.013661
GTCTCTC60300.090.8955152
TCTCTCA66400.082.435993
AGCTGGT69900.078.515749
AGTCTCT76350.072.167651
TCCGATC3700.070.5405356
TCAGCTG79000.069.563297
CAGCTGG85800.064.726118
CTCTCAG88300.062.3187984
CTGATGG125450.062.1263471
GGCTCAA126100.061.4036526
GCTCAAA128300.060.4637577
CTCAAAC130050.059.6501358
TCTCAGC93100.058.950055
GATGGCT132050.058.746693
TCAAACA132750.058.3822989
CTCAGCT96550.057.2190556
TGATGGC139600.055.881092
TGGCTCA143300.054.1346865
TACGGCG1353.6379788E-1253.70376
Produced by FastQC (version 0.11.5)