FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1827847
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAG6500.0123.807691
TACGGGC8200.0122.896341
ACGATAC9200.0115.054351
CGATTGA6750.0109.555561
GCATGCG6700.0107.126861
ATCGACG3250.0107.076929
GGCTCGA4550.0106.758231
CACCGAA3100.0105.2419368
CCTTACG4900.0103.571431
TAAACCG7600.0103.026321
GCGCCAA10400.0101.7788541
ATCGTCG6100.099.836079
ACGAGCC7450.098.288591
CGATACG4650.098.225812
TCGCGAG6700.097.388061
ATCCGCA5900.097.076277
CGCTATC3300.096.6666641
TGACTAG6800.095.955881
ACGTTAG5300.094.3867953
CTCGTAC7300.094.349321
Produced by FastQC (version 0.11.5)