FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2350432
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC429031.825324025540837No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC390111.6597374440102923No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC181510.7722410178214048No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC159010.6765139344597079No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC41170.17515928986671386No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT33650.14316517133871562No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG81450.0103.3425451
GTCTCTC61000.087.594272
TCTCTCA69900.077.167383
TCAGCTG73900.073.5791557
AGTCTCT74850.071.386111
AGCTGGT78050.069.3882149
CAGCTGG84800.064.8909158
TACGGCG1400.062.1428576
GGCTCAA148550.061.1528056
GCTCAAA151050.060.092687
CTCAAAC151000.060.0165528
CTTCCGA6650.057.781964
TCAAACA158450.057.240469
TCCGATC6750.056.9259266
ATGGCTC165300.054.868424
ATACGGC1600.054.3750045
CTGATGG171700.053.0343631
CCGATCT7200.052.361117
TGGCTCA174500.052.1418345
GATGGCT176800.051.2994883
Produced by FastQC (version 0.11.5)