FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047610
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA7200.0115.7986151
CGAAGTG5750.0114.739135
CTTACCG11700.0110.299141
GGCTCGA5900.0109.36441
GTAACGC4350.0108.333338
CGGACGT8050.0106.27331
CGGACTG8600.0106.2209241
TAACGCG4450.0105.898879
ACGATAC6750.0104.185191
ATTACGA4850.0103.144339
CGCTTCA11550.0102.943721
TCGCGAA1850.0101.8918849
CTTTGCG9750.0101.8717961
AACGTTG8500.0101.51
ACGGGTC10650.0100.751181
CATTCGC10550.099.6445541
CGTACGT3450.098.768113
CCTTCGA7650.097.614381
TGCGTAC3200.097.4218755
CTAATCG3350.097.388064
Produced by FastQC (version 0.11.5)