FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047610
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC472052.3053706516377632No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC440192.149774615283184No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC215711.0534720967371716No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC194380.9493018690082584No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC43780.21381024706853355No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT39530.19305434140290387No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG21110.10309580437681004No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG89150.0112.9584961
GTCTCTC72550.086.740182
AGTCTCT74550.084.510391
TCTCTCA75800.082.542883
TCAGCTG79400.079.074317
AGCTGGT82300.075.3189549
CAGCTGG87650.071.466068
GGCTCAA171800.062.7095456
CTCAAAC174000.061.9166688
GCTCAAA174250.061.869447
CTCTCAG103050.061.2081534
TCCGATC4450.060.2808956
TCAAACA183550.059.011179
TGGCTCA187050.057.713185
CTTCCGA4800.057.3958324
GATGGCT190150.056.65793
CTGATGG191500.056.2963451
TCTCAGC112500.056.0022245
ATGGCTC192850.055.7894744
TACGGCG1206.002665E-1154.3756
Produced by FastQC (version 0.11.5)