FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2126813
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC556782.6179076392705896No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC386661.818025374116107No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC119710.5628609567460797No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC81430.382873341473839No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG80800.3799111628525874No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG73150.3439418510231036No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC55260.25982538192121263No Hit
GTTCAGAAATTGGATCACACCTGAAAGTTACATTCTGTCCCCTCTTTGTG40770.1916952736324256No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATGG40040.18826290792843564No Hit
GTCAGAAACTCTGGGCCCTGCCCCAGGGTCTGTCGGTACCAATAAAGGCG39910.1876516647208758No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATG37870.17805984823301343No Hit
GCTTCATTCTGGAAGTAAGTCAGAAACTCTGGGCCCTGCCCCAGGGTCTG37680.17716649277581056No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAAAG33730.1585941030076457No Hit
GTTACATTCTGTCCCCTCTTTGTGATCTTGTGTCTGGGGTCCTGGGAGAC33380.15694844821806148No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT31730.1491903613528787No Hit
GAAATTGGATCACACCTGAAAGTTACATTCTGTCCCCTCTTTGTGATCTT27940.1313702709170952No Hit
GGATCACACCTGAAAGTTACATTCTGTCCCCTCTTTGTGATCTTGTGTCT27920.13127623350054754No Hit
GTCGGTACCAATAAAGGCGGTTGTGTTCAGAAATTGGATCACACCTGAAA27720.13033585933507083No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG27390.12878424196203428No Hit
GCCCTGCCCCAGGGTCTGTCGGTACCAATAAAGGCGGTTGTGTTCAGAAA26470.12445852080084144No Hit
GGTACCAATAAAGGCGGTTGTGTTCAGAAATTGGATCACACCTGAAAGTT26460.12441150209256761No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTC25330.11909838805762424No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTC24230.11392633014750239No Hit
GTCTGGGGTCCTGGGAGACTCCAGTATCTGCGTGATCTGCCCCCAGGAGA24190.11373825531440704No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGGA23540.11068203927660776No Hit
GCCTTGATTTTTCTAGTTGAGCTTCATTCTGGAAGTAAGTCAGAAACTCT23320.1096476276945834No Hit
GATCACACCTGAAAGTTACATTCTGTCCCCTCTTTGTGATCTTGTGTCTG23240.10927147802839271No Hit
GGAAGTAAGTCAGAAACTCTGGGCCCTGCCCCAGGGTCTGTCGGTACCAA21870.10282991499487731No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTGA21510.10113724149701925No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG64150.0114.485581
GTCTCTC93100.0101.702472
AGTCTCT94750.099.778361
AGCTGGT101900.093.204129
TCTCTCA110300.086.237533
TCAGCTG114950.083.316667
CTCAGCT120400.079.6054846
CTCTCAG120300.079.490864
CAGCTGG123650.078.216748
CTGATGG139850.074.599581
CTCAAAC147700.071.665548
TGATGGC148600.070.3532942
GGCTCAA150850.069.688436
GCTCAAA152750.068.963997
GATGGCT151100.068.949373
TCAAACA156850.067.76229
TCTCAGC144800.066.391575
ATGGCTC157500.066.331744
TGGCTCA161050.065.184725
TCCGATC3650.063.5616466
Produced by FastQC (version 0.11.5)