FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32586093
Sequences flagged as poor quality0
Sequence length35-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG483410.14834856084158357No Hit
CGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGG341140.10468883152085769No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA335940.1030930587474847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGCG92350.030.7874628
TGCGCGA101600.029.45555111
CGCGAGT101600.029.3209813
GCGAGTC110200.027.00126614
CGAGTCG111750.026.53362515
GTGCGCG113150.026.47942210
CGCACGA110700.026.15704328
GTCTAAC119200.025.975491
CGTGCGC115500.025.7302119
CGCTCGC176450.025.32935770
TAACACG113050.025.1808784
AACACGT117400.024.4549245
GCTCGCA120550.024.22090525
CTCGCAC120550.024.0772726
GCGCGAG125450.023.96720912
GGGCTCG127800.023.11839123
GCACGAA127900.022.96543729
ACACGTG126150.022.9507946
TCAGACG51150.022.949892
CCGCCGT130850.022.02655438
Produced by FastQC (version 0.11.5)