FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37580503
Sequences flagged as poor quality0
Sequence length35-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG556700.14813532431963458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAC44550.024.727551
CGCACGA36600.022.95728328
TGCGCGA39000.022.16653611
CGCGAGT39100.022.11175713
TATCGCG11950.020.92033822
ACGTGCG44200.019.9504438
TCGCACG43950.019.59240227
CGAGTCG44150.019.26815
GTCGACC58200.019.2158472
CGCTCGC63250.018.84398870
GCGAGTC50200.017.29164714
TCGACCC69150.016.869253
GGGCGAT42350.016.8217051
GGCGATC39450.016.8156262
GCACGAA52750.016.25821329
CCGTGCG69950.015.8819358
GCTCGCA55050.015.70425125
GTGCGCG58850.015.51581910
CGATTTC130200.015.44487969
CTCGCAC56000.015.438416526
Produced by FastQC (version 0.11.5)