FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36195955
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG477690.13197331027735004No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGC439160.1213284744110219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG103650.052.8667770
CCCGATC127600.030.52626469
TACCGGC29850.028.1295370
CGATCGG88700.026.86234570
ACCCGAT137800.026.11301268
CTCGCCG35400.025.41363170
CCCTACG139300.025.35189624
CCTACGT143500.024.73108925
TACGTTC145900.024.39671927
CGATTAG45900.024.1381424
CTACGTT148750.023.92832626
GTTCGAT50250.023.5706941
CACCCGA150550.023.37588567
CCCGTCT120150.020.6698021
CTCGTTT99200.020.6232761
ACGTTCT174350.020.61487228
TCGATTA52600.020.469133
CGTTCGA23800.020.42717
GACTACC179250.020.05009519
ACTACCC182550.019.63066120
Produced by FastQC (version 0.11.5)