FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36195955
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG439110.12131466071277854No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA416480.11506258088783677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA106450.031.1396211
ACGTGCG107350.030.9089628
CGCGAGT107050.030.8701613
GTCTAAC119150.028.9262161
CGAGTCG116150.028.42196515
CGCTCGC171150.028.2182170
GCGAGTC118250.028.0051314
GTGCGCG121450.027.6079810
CGCACGA119600.027.25298328
CGTGCGC122150.027.2223639
TAACACG129000.025.6947294
CTCGCAC127900.025.53904526
TCGCACG128500.025.5009127
GCTCGCA129200.025.4157125
GCGCGAG130850.025.33354812
AACACGT134100.025.1575935
GGGCTCG134750.024.5491223
GCACGAA138700.023.70058829
CCGCCGT140100.023.29423938
ACACGTG148000.022.841316
Produced by FastQC (version 0.11.5)