FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39296946
Sequences flagged as poor quality0
Sequence length35-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGC1023110.26035356538902543No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG719840.18317962927704357No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC572720.1457416054672544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG169050.050.9490870
CGATCGG112900.032.44613670
ACCGCGT23500.029.9961936
CCGCGTT24400.028.7419017
CCCGATC217350.027.53660669
CGCGTTC26500.026.5950498
CCCTACG344250.026.24001724
CCTACGT350750.025.7934325
TACGTTC354550.025.53757327
ACCCGAT216200.025.43901868
CTACGTT361700.025.05102726
ACGTTCT381250.023.73092528
CACCCGA227700.023.71547967
GACTACC388650.023.63507319
ACTACCC393800.023.28208220
ACCCTAC402300.022.73841723
GGTTCTA391700.022.53713466
TACCCTA410600.022.28720322
GCTTGAC412100.022.2238615
ATGGACC403350.022.22021742
Produced by FastQC (version 0.11.5)