FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39335287
Sequences flagged as poor quality0
Sequence length35-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGC694010.17643445692921983No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG592340.1505874356528783No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC398300.10125768244680661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG128000.054.7734470
CGATCGG86700.033.5801170
CCCGATC151750.032.53788869
CCCTACG225100.028.14648824
TACGTTC230150.027.50048427
CCTACGT232800.027.27521125
CTACGTT239900.026.45372226
ACCCGAT174850.025.91260768
ACGTTCT258350.024.6599628
GACTACC268450.023.82080319
CACCCGA187350.023.72939367
TACCGGC19950.023.146370
ACTACCC277500.023.10655220
ACCCTAC278800.022.94902823
CGTTCTC279700.022.66677329
GCTTCGA46300.022.39861969
CTCGCCG29650.022.2214270
ATGGACC281900.022.18992242
CCCGTCT106750.022.1411781
TACCCTA290000.022.06269322
Produced by FastQC (version 0.11.5)