FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39335287
Sequences flagged as poor quality0
Sequence length35-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG859770.21857473672430558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGT26250.024.5892913
TGCGCGA27250.023.81282811
CGCACGA27650.022.71644228
ACGTGCG29750.021.6942548
CGAGTCG30950.020.85521315
CGCTCGC48700.018.28653170
GTGCGCG37000.017.91305710
CGTGCGC38500.017.1250159
GCGAGTC38600.016.99159214
TCGCACG39400.016.55850827
GCGACAT58550.016.4160377
TGCGACA59700.016.157696
CGATTTC137950.016.12947569
GCCACGA58600.015.9304319
CCGCCGT40250.015.8676738
GGCGATC36050.015.3102512
GCTCGCA43000.015.2522825
TGCGATT132300.014.99540467
CTGCGAT131300.014.91499466
CTCGCAC43800.014.81573726
Produced by FastQC (version 0.11.5)