FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36917919
Sequences flagged as poor quality0
Sequence length35-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG506500.137196248791813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGT22000.024.14034713
CGCACGA21550.023.8386128
TGCGCGA23150.022.78950911
ACGTGCG26000.020.29038
CGAGTCG26500.020.04112215
GCGACAT48100.019.1934247
TGCGACA48350.018.878766
CGCTCGC43300.018.24352370
TATCGCG4250.017.9670729
CGTACGT9200.017.41288670
TCGCACG33400.016.62804627
CTGCGAC55100.016.5662045
GTGCGCG32600.016.39628610
CGACATC57050.016.3649568
CCGCCGT32800.016.1942438
GCCACGA53300.015.82603219
GCGAGTC33900.015.76871614
CGTGCGC34600.015.44856559
GCTGCGA60500.015.2024674
GGGCTCG37250.014.53621423
Produced by FastQC (version 0.11.5)