FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36335085
Sequences flagged as poor quality0
Sequence length35-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG543460.149568935919649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGT48150.028.76467913
TGCGCGA48850.028.7059711
ACGTGCG50450.027.794088
CGCACGA51200.026.4406928
CGAGTCG55100.024.88467815
GTGCGCG59150.024.118110
GCGAGTC57450.023.92715514
CGTGCGC59600.023.6448069
CGCTCGC85150.023.59633870
GCGACAT46100.021.8335847
GGGCTCG64200.021.78891423
TGCGACA45600.021.6921186
CTCGCAC64900.021.55476426
TCGCACG63850.021.52875927
GCTCGCA65200.021.45493525
GCGCGAG66500.021.2441612
CCGCCGT64950.021.06018338
GCGTCGA11300.020.27313411
TAACACG70150.020.0875454
CGTCGAT11150.019.92378612
Produced by FastQC (version 0.11.5)