FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35616451
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG1642300.46110714399927155No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA954540.2680053663965565No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTG891660.25035060343322807No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG710470.19947804456990956No Hit
CGCTACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTA699640.19643731488013785No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT614320.17248209261501096No Hit
CGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGG597910.1678746711737225No Hit
CGGAGCCCCGCGGACGCTACGCCGCGACGAGTAGGAGGGCCGCTGCGGTG526200.14774071678281478No Hit
AGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGCGGGCCCGGGTGG476750.13385668325010822No Hit
CCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCA470550.13211591463731184No Hit
CCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACG430830.12096376475017122No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCA411640.11557580512443534No Hit
CTTGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCT410890.1153652282761132No Hit
GTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGC401810.1128158445657598No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGG398730.11195107564198353No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA398440.11186965259396564No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC398290.1118275372243012No Hit
CGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAG383300.10761880794916932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC189450.040.530731
GCCAGTA308900.040.44184
CCAGTAG325800.038.375345
TAGCATA331500.038.237729
CAGTAGC336500.037.494586
GTAGCAT338700.037.2306448
CATATGC345900.036.14537412
GCATATG352150.035.5825211
TGCCAGT359500.034.7626273
GCTTGTC362050.034.25574517
ATATGCT367700.034.00240713
CCTGCCA377950.033.25821
ATGCTTG382800.032.4892915
TATGCTT391700.031.91903114
CTGCCAG393950.031.5224692
TCAGACG155950.031.230012
TCTAACA248200.031.1783812
ACGTGCG256200.029.7992468
TGCGCGA258000.029.61818311
CGCGAGT260500.029.34723313
Produced by FastQC (version 0.11.5)