FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33188300
Sequences flagged as poor quality0
Sequence length35-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG83900.034.79847770
ACCGCGT30750.027.2215796
CCGCGTT31500.026.5733287
CCGACTA27950.025.10253118
CGACTAT28300.024.91458319
CGCGTTC35850.023.3489118
AGTCGTC32200.021.78924232
AGACCGC39300.021.56444
CGTTCGA23450.021.1803257
CCCGATC100400.020.76652569
ACGTTCG24050.020.652016
GCGTACG9950.020.5952192
CAGACCG43850.020.1980153
CAAGTCG34850.020.03241730
GACCGCG41550.019.8952755
AAGTCGT36050.019.17304831
CGTACGG10350.019.1281363
ACCCGAT103250.018.79066868
GAACGAT45800.018.58432245
GTCGTCA37250.018.55586833
Produced by FastQC (version 0.11.5)