FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25179939
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG1147420.4556881571476404No Hit
GTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCG791820.3144646220151685No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA789030.3133565970910414No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC775960.3081659570342883No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC704650.2798457931133193No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC666830.26482589969737413No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC515700.2048058972660736No Hit
CCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGG495970.19697029448721062No Hit
CCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGC493110.1958344696545929No Hit
GCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCG425610.16902741503861468No Hit
CCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGG401490.15944836085583844No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG358380.14232758864110034No Hit
CCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTG319090.12672389714685173No Hit
GCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGG284630.1130383993384575No Hit
GTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTC278360.11054832182079552No Hit
CCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCG270450.10740693216135272No Hit
CCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCAC267040.1060526794763085No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA259230.10295100397185236No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG256100.1017079509207707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACG114650.044.6443372
CGTGGAC167150.043.4141170
CGTACGG121050.042.284043
GGCGTAC123450.041.6315161
CCGCACG61650.032.54266470
GGACGGT91600.031.97976370
GTACGGA162800.031.5257594
TACGGAA165400.031.0091975
ACCGCGT76250.030.5126886
CCGCGTT78200.029.751417
CGCGTTC81650.028.6221778
AGACCGC82050.028.355794
CGGAAGA183800.027.9045147
AGCCCGT179450.027.03005866
TGATCGA186250.026.76334854
CCGTGGA196900.026.5599769
GCGTTCT88200.026.4967659
CCGCTCG195700.026.40347528
AGACCCG194350.026.39017911
CGCTCGT195600.026.20497529
Produced by FastQC (version 0.11.5)