FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25179939
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC2892061.148557190706459No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC2021870.802968585428265No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1577310.6264153380196831No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC1545390.6137385797479493No Hit
CTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCC648610.25758998065880934No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG623790.24773292739112673No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA596450.23687507741778088No Hit
CAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGG482330.1915532837470337No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG343900.1365769789990357No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG311520.12371753561436348No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG294960.11714087154857682No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA289760.11507573548927184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCAG410200.035.7329061
TGCAGGT453000.032.2342643
CTGCAGG459400.031.7724132
AGGTCGA505150.028.614686
GGGGCCC1110050.027.70978770
GGTCGAC794450.026.0481511
ACCGGGG846600.024.58981767
CGGGGCC945600.024.01443569
CACCGGG871250.023.79964366
GCAGGTC636050.022.966884
CCGGGGC940250.022.7108868
CCACCGG947050.021.87183665
GTCGACC956200.021.6158082
TCGACCC972400.021.2812293
CAGGTCG697650.021.0130315
CGACCCG1005100.020.6116754
ACCCGTG1017000.020.3356426
GACCCGT1027150.020.1956235
TTCCGCA39400.019.9329743
CCGTGCG1055800.019.654148
Produced by FastQC (version 0.11.5)