FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39992190
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGC441860.11048657250328127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG102750.043.3370270
CCGCGTT41350.031.653887
ACCGCGT43000.030.3585136
CGCGTTC48200.027.4435758
GACCGCG49800.026.1435225
CCCGATC121700.025.83786869
AGACCGC51650.025.3412674
CAGACCG56250.023.8860073
ACCCGAT129250.022.47867468
CGACTAT35300.022.42447719
CCGACTA35700.022.1732518
GCGTTCT64800.020.573839
CCCTACG188350.020.23905424
CACCCGA143950.019.83869467
GAACGAT62550.019.71179445
TACGTTC194550.019.6296627
AGTCGTC39500.019.33742532
ACGTTCG24850.019.28036
CCTACGT197200.019.27796725
CTACGTT200400.019.09142926
Produced by FastQC (version 0.11.5)