FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39992190
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG754250.18859932401801452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGT65500.027.46238313
ACGTGCG67000.026.9512128
TGCGCGA66900.026.88768411
CGAGTCG72850.024.7393415
CGCACGA70100.024.62068228
CGCTCGC100550.024.59817570
GCGAGTC79000.022.98925614
GTGCGCG85850.021.47850410
GGCGATC43150.021.0957832
GCTCGCA84750.020.93800425
TCGCACG83700.020.91048227
CGTGCGC87500.020.835369
CTCGCAC84900.020.4922226
TAACACG91550.020.0659684
GCACGAA89300.019.59972629
GTCTAAC107050.019.2934911
GGGCTCG92850.019.11137823
AACACGT97850.019.022015
CCGCCGT92800.018.75075538
CGCAATG93900.018.57475547
Produced by FastQC (version 0.11.5)