FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29817788
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG10150.030.62850670
TCGCGCG24700.023.717860
GCGCGTC25400.023.09880862
CGCGTCC26400.021.9773763
CCCGTCT72350.021.4121861
CGCGCGT27650.021.20285661
GTCCGAT8100.020.5375331
CTCGCGC29850.019.61287759
CCCCACT179050.019.1431751
GTCCGTT13150.018.9757421
CCTCGCG31400.018.9668958
GCGTCCT32150.017.95374764
GCAGTCG84300.017.67769420
CAGTCGA89900.017.34761421
AGTCGAG90450.017.01211722
CTCCGCT23050.016.990241
GTCGAGT90200.016.94403323
CGCGGGC34650.016.80323821
CCCTACG86450.016.67675824
CGTCTCT79900.016.1790353
Produced by FastQC (version 0.11.5)