FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29817788
Sequences flagged as poor quality0
Sequence length35-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1272060.4266111221932358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAC32300.023.3264061
TCGCGCG9600.019.98460
GTCGACC40950.018.2993742
CGACCCG41650.018.1506794
TCGACCC42850.017.8056933
CCGTGCG45700.016.5418348
CGTGCGG45950.016.5273029
GACCCGT48400.015.9056615
ACTCGAC17250.014.74424358
GTGCGGA53250.014.587193510
CGCGCGA50450.014.43633234
ACCCGTG53350.014.42985256
AGGTCGA34850.014.4280826
ACGCGCG50950.014.36229533
GACGCGC51700.014.22095532
GGACGCG53850.013.97499431
CACCGGG55450.013.79589266
CTCGCGC13900.013.793406559
GCGCGTC13800.013.66909462
TGCGGAG58100.013.488873511
Produced by FastQC (version 0.11.5)