FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12910408
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATTA86500.022.20836412
GGATTAC79850.021.9544813
GATTACA88500.020.40227314
ATTACAG97800.018.63934315
TGGGATT126100.017.28754211
TTACAGG106700.017.01889216
TACAGGC93600.015.47585117
AAAGTGC99600.014.6106594
CCAAAGT114950.013.6946032
CTGGGAT163900.013.407083510
GCTATAT19250.013.0879671
ACCGCGC26850.012.90117731
ACAGGCG64350.012.83222318
CAAAGTG120700.012.7225133
GCGTGAG58000.012.608622
GGCGTGA55100.012.57357521
AAGTGCT117700.012.5424855
CTACTCG17450.012.028232
CGTGAGC57200.011.98952323
CCCAAAG158300.011.87035751
Produced by FastQC (version 0.11.5)