FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9170642
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA9700.032.8441281
GGATTAC54100.024.45426813
GGGATTA62500.023.96743412
GATTACA57900.023.39335814
CGTCGAG14600.023.014412
ATTACAG65750.020.33423215
TGGGATT87550.019.14860511
TTACAGG72000.018.37466616
GTCGAGA17600.018.2960193
TACAGGC67050.017.85202617
AAAGTGC67900.016.654
CCAAAGT78650.015.6203192
CTGGGAT121300.014.65752810
AAGTGCT78900.014.6385195
ACAGGCG48450.014.5198618
CAAAGTG86350.014.3895593
CACGCGA9600.014.21841422
GGCGTGA42100.014.04955121
GCGTGAG41850.013.71532822
TGATCAA42650.013.6221899
Produced by FastQC (version 0.11.5)