FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11584087
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA14200.031.556811
GGATTAC76000.021.73639113
GGGATTA82250.020.6803312
CGTCGAG23400.020.047511
GATTACA85250.019.5831714
TGGGATT115250.016.94534311
ATTACAG98600.016.93169615
GTCGAGA28350.016.6670382
TACAGGC91300.015.60208417
TTACAGG106000.015.38647216
AAAGTGC97200.013.6835514
GACTTGT77550.013.5852043
CTGGGAT150400.013.40391710
CGAGTAG16200.013.3954773
TGATCAA79100.013.27411659
TCGAGAC36950.013.2615743
ACTTGTG85800.013.2171934
ACAGGCG62800.012.929666518
GTGATCA83950.012.7990748
GATCAAG84000.012.79134610
Produced by FastQC (version 0.11.5)