FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11584087
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATTA85800.019.12691312
GGATTAC80800.018.66496313
GATTACA89450.017.3690114
ATTACAG102200.015.6117915
TACAGGC88300.014.66160817
TGGGATT120350.014.65358211
TTACAGG107900.014.39806116
CGGCTAA11550.012.4234439
CTGGGAT146550.012.32024910
ACAGGCG59350.012.26272418
AAAGTGC101150.012.1037764
AGTAGCT55950.011.8165775
CCAAAGT112500.011.7536052
GGCGTGA51050.011.65232421
GCGTGAG53950.011.28540522
ACCGCGC22500.011.19685831
CAAAGTG120300.011.0781383
AAGTGCT113400.010.9816325
CCCAAAG151600.010.9633311
AGGCGTG58500.010.94597820
Produced by FastQC (version 0.11.5)