FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37147495
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC376350.024.49566313
GGGATTA404750.024.18622212
ACGTCGA34750.023.9780451
GATTACA402550.023.24038514
TGGGATT491400.021.41000711
ATTACAG438400.021.14029315
TTACAGG451550.019.90454316
TACAGGC386550.019.10470617
CGTCGAG62700.018.7613181
AAAGTGC384450.018.4540674
CTGGGAT582100.018.06197710
ACAGGCG250050.017.14639518
GCGTGAG223450.017.04155322
GGCGTGA221100.017.0326821
ACCGCGC108150.016.95789131
CCAAAGT440200.016.8165452
CGTGAGC230150.016.63669223
AAGTGCT432500.016.5165275
CAAAGTG449800.015.9363433
AGGCGTG246400.015.8803720
Produced by FastQC (version 0.11.5)