FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10555608
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA9700.025.9843241
GGATTAC63450.025.26376513
GGGATTA71900.024.53374312
GATTACA68600.023.57111214
ATTACAG79850.019.4612315
TGGGATT106300.018.43813311
TTACAGG89700.017.20712516
CGTCGAG14900.017.1476632
TACAGGC73700.016.71619217
AAAGTGC80150.016.4193044
CCAAAGT93750.016.1653442
CAAAGTG97300.014.9281393
GTCGAGA16650.014.9250073
CTGGGAT137850.014.39588810
AAGTGCT93050.014.2180385
CCCAAAG130800.013.3014591
ACAGGCG51850.012.89278718
GGCGTGA47100.012.55821721
GCGTGAG46900.012.53720422
CGTGAGC49150.012.31932323
Produced by FastQC (version 0.11.5)