FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7820443
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATTA57150.025.22413812
GGATTAC52600.023.2153413
GATTACA54600.022.55850814
ATTACAG61100.019.46994615
TGGGATT79950.019.03733411
TTACAGG65950.017.4546416
AAAGTGC61250.016.7896044
TACAGGC57000.016.45069317
CCAAAGT70000.015.1423692
CTGGGAT105700.014.49878810
CAAAGTG75600.014.157813
AAGTGCT73000.014.1354465
CGGCTAA6700.014.10413939
GCGTGAG35850.013.66432422
CGAGTAG11350.013.2516223
CCCAAAG97250.013.2052061
GGCGTGA35200.013.12140921
ACAGGCG40550.012.85681918
CGTGAGC37250.012.77494723
CCTATAT12900.012.2065091
Produced by FastQC (version 0.11.5)