FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8214847
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA8100.026.3657651
GGATTAC49350.024.53874213
GGGATTA54250.023.8060512
GATTACA56600.021.2100214
ATTACAG64800.018.58004815
TGGGATT79050.018.5513511
CGTCGAG12250.017.7142032
TTACAGG66150.017.19558116
TACAGGC58350.016.91508717
CTGGGAT101300.014.85670110
GTCGAGA15950.014.2632563
GCTATAT10600.014.2022831
GGCGTGA35500.013.999763521
AAAGTGC63350.013.9778364
CCAAAGT71200.013.5181932
CGAGTAG10950.013.4245943
ACAGGCG41750.013.24544718
GCTAATT27650.012.65801441
CGGCTAA8450.012.42582639
CAAAGTG78250.012.2555333
Produced by FastQC (version 0.11.5)