FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24162985
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATTA216650.022.19734212
GGATTAC203500.022.1868913
GATTACA210450.021.919414
ACGTCGA25000.021.842461
ATTACAG225000.020.26886615
TGGGATT269000.019.30876511
TTACAGG247050.018.27563316
TACAGGC226650.017.20278717
AAAGTGC215700.017.0222234
CCAAAGT240750.015.716172
CGTCGAG43850.015.4065241
CTGGGAT352000.015.00449310
AAGTGCT251350.014.9699845
GGCGTGA149600.014.48193521
ACAGGCG170050.014.44873418
CAAAGTG257200.014.4252713
TGATCAA111250.014.0317349
GCGTGAG159300.013.92953222
GTCGAGA48800.013.9145522
AGGCGTG165650.013.6915320
Produced by FastQC (version 0.11.5)