FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36476262
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC227500.024.15342713
GGGATTA262700.023.62152912
GATTACA247300.021.9367214
ATTACAG278100.019.21773115
TGGGATT369350.018.24088911
TTACAGG294600.017.67816716
AAAGTGC268200.016.5189044
TACAGGC262600.016.14014817
CCAAAGT319050.015.1609522
CAAAGTG334650.013.9608063
CTGGGAT497100.013.87694510
AAGTGCT318550.013.8752185
ACAGGCG190100.013.09021618
CCCAAAG455100.012.7259921
GGCGTGA168650.012.7008321
GCGTGAG176300.012.28870222
CGTGAGC183600.011.81909723
AGGCGTG192450.011.62109420
GCTATAT43900.011.4857921
CAGGCGT186750.011.43229219
Produced by FastQC (version 0.11.5)