FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27666231
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATTA270300.023.47587212
GGATTAC256250.022.9463213
GATTACA266800.022.16978314
ACGTCGA27450.020.657921
ATTACAG291050.020.16647315
TGGGATT342800.019.71573611
TTACAGG311350.018.53682916
TACAGGC272100.018.13676317
ACCGCGC83850.017.32264531
AAAGTGC273700.017.3026184
GGCGTGA164350.016.88781421
ACAGGCG190700.016.59154918
CGTCGAG43250.016.5104161
CTGGGAT421700.016.03534110
AGGCGTG178150.016.03147520
CCAAAGT305250.015.984242
CGTGAGC179300.015.85048623
GCGTGAG174250.015.80770322
AAGTGCT308300.015.5311555
CAGGCGT180000.015.49726319
Produced by FastQC (version 0.11.5)