FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27666231
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATTA292250.023.37685412
GGATTAC272150.023.3158813
GATTACA281600.022.70760514
ATTACAG305450.020.83143615
TGGGATT357150.020.15749511
TTACAGG326250.019.31047416
TACAGGC286200.019.05285817
AAAGTGC287550.018.0485464
ACAGGCG193900.016.94920518
ACCGCGC89750.016.84642231
CCAAAGT322300.016.6802232
AAGTGCT318850.016.56225
CTGGGAT444600.016.52323510
GGCGTGA171750.016.46581521
CAAAGTG336350.015.7631683
GCGTGAG180650.015.596477522
CGTGAGC183000.015.58728223
AGGCGTG190100.015.2444920
CAGGCGT189750.014.8852619
CCCAAAG412150.014.7827931
Produced by FastQC (version 0.11.5)