FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17820814
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC175500.024.70948413
GGGATTA190900.024.5863912
GATTACA191200.022.91816714
ACGTCGA16300.022.3336811
TGGGATT235500.021.10410311
ATTACAG210200.021.04651515
TTACAGG216150.019.85185416
TACAGGC183750.019.37149417
CGTCGAG27350.019.3256531
AAAGTGC186150.018.5584584
GGCGTGA101450.018.3193821
ACAGGCG116700.018.2348518
CTGGGAT282800.017.69817710
CCAAAGT210050.017.4298292
GCGTGAG106550.017.34388422
CGTGAGC107050.017.1647923
AGGCGTG114300.016.96413820
ACCGCGC52250.016.94752131
AAGTGCT208600.016.7457285
CAAAGTG213650.016.726623
Produced by FastQC (version 0.11.5)