FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18510614
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC218500.024.21907213
GGGATTA238500.023.71428912
GATTACA238650.022.1303614
TGGGATT283800.021.28529511
ATTACAG261100.020.09349315
TACAGGC217200.020.02942317
TTACAGG258850.019.84910416
ACAGGCG122000.019.53655418
CTGGGAT310350.019.10319110
ACCGCGC51300.018.2842931
GCGTGAG102850.018.2401422
AAAGTGC218750.017.8539124
CGTGAGC107250.017.65490323
AGGCGTG114900.017.2408820
CAGGCGT112200.017.03193519
GGCGTGA109750.016.96577321
CTACTCG37200.016.6535282
CGGCTAA31750.016.53573439
CGAGTAG41250.016.458863
AAGTGCT235750.016.4477945
Produced by FastQC (version 0.11.5)