FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37953965
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA35750.026.33871
GGATTAC221850.022.749513
GGGATTA243800.021.83559212
GATTACA240500.021.34894814
ATTACAG282600.017.72275415
CGTCGAG55500.017.0909352
TGGGATT353250.017.0022911
TTACAGG306900.016.2510616
TACAGGC269850.015.96616117
AAAGTGC291700.014.4353674
CCAAAGT334500.013.5298162
GTCGAGA69450.013.5068923
CTGGGAT467700.013.25345810
ACAGGCG186200.012.78187818
CAAAGTG359600.012.3421973
CGAGTAG58850.012.2521893
AAGTGCT347200.012.2186835
CCCAAAG460650.012.1809321
TGATCAA189350.011.7933259
ACCGCGC77600.011.77196131
Produced by FastQC (version 0.11.5)