FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30832416
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA35100.028.9207021
CGTCGAG55000.020.2386881
GGGATTA225100.020.15113412
GGATTAC211850.020.08974513
GATTACA238500.018.24084914
ATTACAG273400.016.10455115
TGGGATT311600.016.05120511
GTCGAGA71550.015.9476362
TACAGGC240050.015.64465317
TTACAGG286100.014.99817516
GACTTGT202450.013.6238633
ACAGGCG163000.013.61351618
TGATCAA204400.013.4593149
AAAGTGC261550.013.3826274
TCGAGAC90300.013.1790173
CTGGGAT391050.013.05870210
CGAGTAG50500.013.0304173
CGAGACT132750.012.9205041
ACTTGTG227800.012.8915264
GGCGTGA141650.012.7497121
Produced by FastQC (version 0.11.5)