FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24669295
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC220200.022.82413513
GGGATTA242050.022.55675912
GATTACA239200.020.92351514
TGGGATT307300.019.3273411
ATTACAG255350.019.27122315
TTACAGG267500.018.0297116
TACAGGC241150.017.41618217
AAAGTGC232950.016.1498874
ACAGGCG150700.016.14110218
CTGGGAT378700.015.77578510
CCAAAGT268700.015.4749662
GGCGTGA128950.015.03676421
AAGTGCT262900.014.7891955
CAAAGTG277500.014.4655423
GCGTGAG135550.014.4595422
CGTGAGC134050.014.30791123
ACCGCGC66350.014.29536331
AGGCGTG146500.014.04762920
CGGCTAA32050.013.97831939
CAGGCGT145850.013.87026519
Produced by FastQC (version 0.11.5)